DNA Ladder Sizing

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

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E-Gel™ Sizing DNA Ladder

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
Size ranges from 50bp to 1,500 bp	Prepare a DNA ladder: For E-Gel™ SizeSelect™ II Agarose Gels, mix and use 25 µL of the ladder without dilution. For E-Gel™ EX Agarose Gels, mix and use 20 µL of the ladder without dilution.

Upstream tips
Size ranges from 50bp to 1,500 bp
Protocol tips
Prepare a DNA ladder: For E-Gel™ SizeSelect™ II Agarose Gels, mix and use 25 µL of the ladder without dilution. For E-Gel™ EX Agarose Gels, mix and use 20 µL of the ladder without dilution.

Manufacturer protocol

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