DNA Ladder Supercoiled

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

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Supercoiled DNA Ladder

New England BioLabs

Upstream tips	Protocol tips	Downstream tips
Size range: 2 kb to 10 kb	Distilled water (dH20)* or TE Buffer-4 μl Gel Loading Dye, Purple (6X), no SDS-1 μl DNA Ladder/Marker-1 μl Total Volume-6 μl

Upstream tips
Size range: 2 kb to 10 kb
Protocol tips
Distilled water (dH20)* or TE Buffer-4 μl Gel Loading Dye, Purple (6X), no SDS-1 μl DNA Ladder/Marker-1 μl Total Volume-6 μl

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