Found 1 discussion for this experiment
2 years ago
2 years ago by Jody Hancock
I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?
Found 1 matching solution for this experiment
|According to the predicted CpG islands and published literature, we developed one pair of nested MSP primers that is listed in Supplemental Table . Genomic DNA of ovarian cancer cells was extracted using a DNA Isolation Kit (Tiangen Biotech, Beijing, China). Bisulfite modification of DNA was performed following the recommended procedures of the BisulFlash DNA Modification Kit (Epigentek, Farmingdale, NY,USA). Placenta DNA was modified with the CpG Methyltransferase (M.SssI, NewEngland Biolabs, Ipswitch, MA, USA) and used as a positive control.|