No discussions found
Found 1 matching solution for this experiment
|Quantification of nuclear DNMT3A and DNMT3B enzyme concentrations were performed as per the manufacturer's guidelines (Epiquik DNMT3A/B Assay Kit, Epigentek, New York). All samples were assayed in duplicate, and various dilutions of the respective DNMT standards were used to plot a standard curve. Microplates were scanned at 450 nm with a reference wavelength of 655 nm. Intra‐assay coefficient of variations for DNMT3A and DNMT3B were calculated as 3.95% and 9.92%, respectively.