DNA methylation profiling Whole genome profiling - mouse liver tissue

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

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Found 3 matching solutions for this experiment

Imprint® Methylated DNA Quantification Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Protocol tips
Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Manufacturer protocol Publication protocol 1 Relevant paper

EZ DNA Methylation kit

Zymo Research

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Manufacturer protocol Publication protocol 1 Relevant paper

EpiTect Bisulfite Kit

Qiagen

Upstream tips	Protocol tips	Downstream tips
	For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Manufacturer protocol Publication protocol 1 Relevant paper

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