DNA quantification Brain tissue

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

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Found 3 matching solutions for this experiment

Protocol tips
Place aluminum foil over
the sample to protect it from light and incubate at room temperature for 5-10 minutes.

Optimize incubation time to
minimize signal bleeding through to other channels.
Protocol tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to
prevent solutions from sticking to the surface
Qubit dsDNA HS Assay Kit

Thermo Fisher Scientific

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.
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