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|Plasmid transfections of 3×105 OVCAR3 cells seeded in 6-well plates were performed in antibiotic-free growth medium using FuGENE HD (Roche Diagnostic Corporation; Indianapolis, IN). Controls were treated with 100 ul/ml OPTI-MEM only and vehicle controls with 3ul/ml FuGENE HD
|The ovarian carcinoma cells were treated with RhoC-expressing plasmid by Attractene Transfection Reagent (QIAGEN) with pEGFP-N1 as a mock or RhoC siRNA (Sigma, USA) by HiPerFect Transfection Reagent (QIAGEN).
|Cells starved of glucose and/or glutamine for 24–36 hr were stimulated as indicated. Cells were transfected with JetPEI Polyplus reagent (Genycell Biotech) and treated as indicated 24 hr later.
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