DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

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Found 3 matching solutions for this experiment

Upstream tips
SuperFect–DNA complex formation should be performed in the absence of serum, antibiotics, and proteins.
Upstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store
in the original glass vials.
Lipofectamine® LTX Reagent

Thermo Fisher Scientific

Protocol tips
Do not add antibiotics to the Transfection Medium
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