DNA transfection Mammalian cells - Primary cells HUVEC

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

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Found 3 matching solutions for this experiment


Polyplus transfections

Protocol tips
jetPEI-HUVEC/DNA mixture was added drop-wise onto the M199M+Glutamax™ I medium (GIBCO) containing 2% FBS, mixed gently, and incubated in a humidified 37°C incubator for 4 h. The growth medium was then replaced and the cells were incubated further for 24 h.
Downstream tips
To verify the transfection efficiency, HUVEC was transfected with Thy-1-pCMS-EGFP (enhanced green fluorescent protein)-C1, and then monitored using an inverted fluorescent microscope.

Targeting Systems

Protocol tips
There are two transfection protocols for transfecting endothelial cells, one protocol uses the Targefect-HUVEC (Targefect F-2) reagent along with the VIrofect enhancer and is used for transfecting cells in presence of serum. These protocls are applicable

The second protocol uses the Targefect -HUVEC (Targefect F-2) reagent along with the Peptide enahncer and requires that cell be transfected in the absence fo serum.
Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.
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