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|jetPEI-HUVEC/DNA mixture was added drop-wise onto the M199M+Glutamax™ I medium (GIBCO) containing 2% FBS, mixed gently, and incubated in a humidified 37°C incubator for 4 h. The growth medium was then replaced and the cells were incubated further for 24 h.|
|To verify the transfection efficiency, HUVEC was transfected with Thy-1-pCMS-EGFP (enhanced green fluorescent protein)-C1, and then monitored using an inverted fluorescent microscope.|
|There are two transfection protocols for transfecting endothelial cells, one protocol uses the Targefect-HUVEC (Targefect F-2) reagent along with the VIrofect enhancer and is used for transfecting cells in presence of serum. These protocls are applicable
The second protocol uses the Targefect -HUVEC (Targefect F-2) reagent along with the Peptide enahncer and requires that cell be transfected in the absence fo serum.