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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-The entire kit may be stored at -20°C for long term storage before reconstitution.
-Avoid repeated freeze-thaw cycles.
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Prepare fresh reagents immediately prior to use. If crystals have formed in the concentrate, mix gently until the crystals have completely
dissolved. |
-Prepare serially diluted standards immediately prior to use.
-Always prepare a fresh set of standards for every use.
-Read plate immediately
after adding STOP solution. |
-Store all reagents as
recommended.
-Any remaining standard should be stored at -20°C after reconstitution and used within 30 days. |
| Upstream tips |
-The entire kit may be stored at -20°C for long term storage before reconstitution.
-Avoid repeated freeze-thaw cycles.
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Prepare fresh reagents immediately prior to use. If crystals have formed in the concentrate, mix gently until the crystals have completely
dissolved. |
| Protocol tips |
-Prepare serially diluted standards immediately prior to use.
-Always prepare a fresh set of standards for every use.
-Read plate immediately
after adding STOP solution. |
| Downstream tips |
-Store all reagents as
recommended.
-Any remaining standard should be stored at -20°C after reconstitution and used within 30 days. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use.
-Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use.
-Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the complete kit at 2-8°C.
-All reagents need to be brought to room temperature prior to use. |
-Do not make foam when diluting the sample.
-Substrate Solution should remain colourless until added to the plate. Keep Substrate
Solution protected from light.
-Stop Solution should remain colourless until added to the plate. |
-Opened reagents are stable 3 months when stored at 2-8°C. |
| Upstream tips |
-Store the complete kit at 2-8°C.
-All reagents need to be brought to room temperature prior to use. |
| Protocol tips |
-Do not make foam when diluting the sample.
-Substrate Solution should remain colourless until added to the plate. Keep Substrate
Solution protected from light.
-Stop Solution should remain colourless until added to the plate. |
| Downstream tips |
| -Opened reagents are stable 3 months when stored at 2-8°C. |
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