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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-The entire kit may be stored at -20°C for long term storage before reconstitution.
- Avoid repeated freeze-thaw cycles. Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Prepare fresh reagents immediately prior to use. If crystals have formed in the concentrate, mix gently until the crystals have completely
dissolved. |
-Before using the kit, briefly centrifuge the tubes in case any of the contents are trapped in the lid.
-Do not let the 96-well plate dry out as this will inactivate active components on plate.
-It is suggested that the diluted ABC and TMB solution be pre-warmed in 37°C for 30 minutes before using. |
-Any remaining standard should be stored at -20°C after reconstitution and used within 30 days. |
| Upstream tips |
-The entire kit may be stored at -20°C for long term storage before reconstitution.
- Avoid repeated freeze-thaw cycles. Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Prepare fresh reagents immediately prior to use. If crystals have formed in the concentrate, mix gently until the crystals have completely
dissolved. |
| Protocol tips |
-Before using the kit, briefly centrifuge the tubes in case any of the contents are trapped in the lid.
-Do not let the 96-well plate dry out as this will inactivate active components on plate.
-It is suggested that the diluted ABC and TMB solution be pre-warmed in 37°C for 30 minutes before using. |
| Downstream tips |
| -Any remaining standard should be stored at -20°C after reconstitution and used within 30 days. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. The
color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.
|
|
| Upstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. The
color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.
|
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