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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| -Allow the microtiter plates to equilibrate to room temperature before opening the foil bags. |
-Run a standard curve with each assay.
-Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
Do not cover plate with foil. |
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity.
-Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days. |
| Upstream tips |
| -Allow the microtiter plates to equilibrate to room temperature before opening the foil bags. |
| Protocol tips |
-Run a standard curve with each assay.
-Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
Do not cover plate with foil. |
| Downstream tips |
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity.
-Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.
|
|
| Upstream tips |
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.
|
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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