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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use. |
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution. |
-Store your standard at <-70ºC after reconstitution, others at 4ºC. |
| Upstream tips |
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use. |
| Protocol tips |
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution. |
| Downstream tips |
| -Store your standard at <-70ºC after reconstitution, others at 4ºC. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents and samples to room temperature (18–25 °C)
before use. |
-Wash by filling each well with Wash Buffer (300 µl) using a
multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
-Read at 450 nm immediately after adding STOP solution. |
|
| Upstream tips |
-Bring all reagents and samples to room temperature (18–25 °C)
before use. |
| Protocol tips |
-Wash by filling each well with Wash Buffer (300 µl) using a
multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
-Read at 450 nm immediately after adding STOP solution. |
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