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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C. |
-Include a standard curve each time the assay is performed.
-Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents.
-In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay.
-Consistent, thorough washing of each well is critical.
|
|
| Upstream tips |
| All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C. |
| Protocol tips |
-Include a standard curve each time the assay is performed.
-Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents.
-In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay.
-Consistent, thorough washing of each well is critical.
|
| Upstream tips |
Protocol tips |
Downstream tips |
--DO NOT FREEZE Streptavidin-HRP.
-Bring all reagents to room temperature before use. |
- Wash Buffer should be dispensed forcefully and removed completely from the wells by aspiration or decanting. Remove any remaining Wash Buffer by inverting the plate and blotting it against clean paper towels.
-Avoid microbial contamination of reagents and buffers. This may interfere with the sensitivity of the assay.
- Determine the optical density of each well immediately after adding STOP solution. |
|
| Upstream tips |
--DO NOT FREEZE Streptavidin-HRP.
-Bring all reagents to room temperature before use. |
| Protocol tips |
- Wash Buffer should be dispensed forcefully and removed completely from the wells by aspiration or decanting. Remove any remaining Wash Buffer by inverting the plate and blotting it against clean paper towels.
-Avoid microbial contamination of reagents and buffers. This may interfere with the sensitivity of the assay.
- Determine the optical density of each well immediately after adding STOP solution. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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