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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-All components of this kit except the standard are stable at 4˚C.
The standard must be stored at or below -20˚C.
-Bring all reagents except the
standard and assay buffer to
room temperature for at least
30 minutes prior to opening. |
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation.
-Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results. |
|
| Upstream tips |
-All components of this kit except the standard are stable at 4˚C.
The standard must be stored at or below -20˚C.
-Bring all reagents except the
standard and assay buffer to
room temperature for at least
30 minutes prior to opening. |
| Protocol tips |
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation.
-Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents and samples to room temperature (18–25 °C)
before use. |
-Wash by filling each well with Wash Buffer (300 µl) using a
multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
-Read at 450 nm immediately after adding STOP solution. |
|
| Upstream tips |
-Bring all reagents and samples to room temperature (18–25 °C)
before use. |
| Protocol tips |
-Wash by filling each well with Wash Buffer (300 µl) using a
multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
-Read at 450 nm immediately after adding STOP solution. |
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