No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
|
| Upstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C.
-Don’t store the diluted HRP-Streptavidin solution for next day use. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C.
-Don’t store the diluted HRP-Streptavidin solution for next day use. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Buffer Concentrates should be brought to room temperature and should be diluted before starting the test procedure.
-If crystals have formed in the Buffer Concentrates, warm them gently until they have completely dissolved. |
-The standard has to be used immediately after reconstitution and cannot be stored.
-The Biotin-Conjugate should be used within 30 minutes after dilution.
- Do not allow wells to dry after washing steps. |
|
| Upstream tips |
-Buffer Concentrates should be brought to room temperature and should be diluted before starting the test procedure.
-If crystals have formed in the Buffer Concentrates, warm them gently until they have completely dissolved. |
| Protocol tips |
-The standard has to be used immediately after reconstitution and cannot be stored.
-The Biotin-Conjugate should be used within 30 minutes after dilution.
- Do not allow wells to dry after washing steps. |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!