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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Make sure the heat block/water bath and microplate reader are switched on before starting the experiment. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Make sure the heat block/water bath and microplate reader are switched on before starting the experiment. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Pre-warm all reagents to room temperature prior to setting up the assay. |
-Do not let wells dry before proceeding to the next step. If an automated machine is used for the assay, use a gentle wash program for all washing steps.
-For best result all additions should be completed within one hour.
-Remove any air bubble formed in the well after acidification of substrate solution because bubbles interfere with spectrophotometric readings. |
|
| Upstream tips |
| -Pre-warm all reagents to room temperature prior to setting up the assay. |
| Protocol tips |
-Do not let wells dry before proceeding to the next step. If an automated machine is used for the assay, use a gentle wash program for all washing steps.
-For best result all additions should be completed within one hour.
-Remove any air bubble formed in the well after acidification of substrate solution because bubbles interfere with spectrophotometric readings. |
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