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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-The entire kit may be stored at -20°C for long term storage before reconstitution.
- Avoid repeated freeze-thaw cycles. Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Prepare fresh reagents immediately prior to use. If crystals have formed in the concentrate, mix gently until the crystals have completely
dissolved. |
-Before using the kit, briefly centrifuge the tubes in case any of the contents are trapped in the lid.
-Do not let the 96-well plate dry out as this will inactivate active components on plate.
-It is suggested that the diluted ABC and TMB solution be pre-warmed in 37°C for 30 minutes before using. |
-Any remaining standard should be stored at -20°C after reconstitution and used within 30 days. |
| Upstream tips |
-The entire kit may be stored at -20°C for long term storage before reconstitution.
- Avoid repeated freeze-thaw cycles. Equilibrate all reagents to room temperature (18-25°C) prior to use.
-Prepare fresh reagents immediately prior to use. If crystals have formed in the concentrate, mix gently until the crystals have completely
dissolved. |
| Protocol tips |
-Before using the kit, briefly centrifuge the tubes in case any of the contents are trapped in the lid.
-Do not let the 96-well plate dry out as this will inactivate active components on plate.
-It is suggested that the diluted ABC and TMB solution be pre-warmed in 37°C for 30 minutes before using. |
| Downstream tips |
| -Any remaining standard should be stored at -20°C after reconstitution and used within 30 days. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
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