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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.
|
|
| Upstream tips |
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.
|
| Upstream tips |
Protocol tips |
Downstream tips |
| -All ELISA reagents must be at room temperature (20-25°C) before use. |
-Minimize lag time between wash steps to ensure the plate does not become completely dry during the assay.
-Use a new adhesive plate cover for each incubation step.
-Take care not to contaminate the TMB Substrate. Do not expose TMB Substrate
solution to glass, foil, or metal. If the solution is blue before use, DO NOT USE IT. |
|
| Upstream tips |
| -All ELISA reagents must be at room temperature (20-25°C) before use. |
| Protocol tips |
-Minimize lag time between wash steps to ensure the plate does not become completely dry during the assay.
-Use a new adhesive plate cover for each incubation step.
-Take care not to contaminate the TMB Substrate. Do not expose TMB Substrate
solution to glass, foil, or metal. If the solution is blue before use, DO NOT USE IT. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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