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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
|
| Upstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours. |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours. |
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
| Upstream tips |
Protocol tips |
Downstream tips |
- Avoid repeated freeze-thaw cycles.
-Prior to assay, the frozen sample should be brought to room temperature slowly
and mixed gently.
-Do not use heat-treated specimens. |
-Bring all kit components and samples to room temperature (20-25 °C) before use.
-Avoid sunlight for Substrate Solution.
-Read absorbance immediately after adding the stop solution.
-TMB is light sensitive, avoid prolonged exposure to light. Also avoid contact between TMB and metal, otherwise color may develop.
|
|
| Upstream tips |
- Avoid repeated freeze-thaw cycles.
-Prior to assay, the frozen sample should be brought to room temperature slowly
and mixed gently.
-Do not use heat-treated specimens. |
| Protocol tips |
-Bring all kit components and samples to room temperature (20-25 °C) before use.
-Avoid sunlight for Substrate Solution.
-Read absorbance immediately after adding the stop solution.
-TMB is light sensitive, avoid prolonged exposure to light. Also avoid contact between TMB and metal, otherwise color may develop.
|
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