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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-All components of this kit except the standard are stable at 4˚C.
The standard must be stored at or below -20˚C.
-Bring all reagents except the
standard and assay buffer to
room temperature for at least
30 minutes prior to opening. |
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation.
-Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results. |
|
| Upstream tips |
-All components of this kit except the standard are stable at 4˚C.
The standard must be stored at or below -20˚C.
-Bring all reagents except the
standard and assay buffer to
room temperature for at least
30 minutes prior to opening. |
| Protocol tips |
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation.
-Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours. |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours. |
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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