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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
|
| Upstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Reagents with different lot numbers should not be mixed.
-All reagents need to be
brought to room temperature prior to use |
-Substrate Solution should remain colourless until added to the plate. Keep Substrate
Solution protected from light .
-Stop Solution should remain colourless until added to the plate. The colour developed in
the wells will turn from blue to yellow immediately after the addition of the Stop Solution.
Wells that are green in colour indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.
|
|
| Upstream tips |
-Reagents with different lot numbers should not be mixed.
-All reagents need to be
brought to room temperature prior to use |
| Protocol tips |
-Substrate Solution should remain colourless until added to the plate. Keep Substrate
Solution protected from light .
-Stop Solution should remain colourless until added to the plate. The colour developed in
the wells will turn from blue to yellow immediately after the addition of the Stop Solution.
Wells that are green in colour indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.
|
| Upstream tips |
Protocol tips |
Downstream tips |
| -Maintain the unopened kit at 2-8 C. |
-Vigorous washing and complete removal of all liquid by aspiration at the end of each washing step is very important to obtain low background values.
-Dilute the reagents immediately before use. |
-Do not store diluted solutions.
- Maintain the BDNF Standard, at 2-8 C for up to 30 days after reconstitution. |
| Upstream tips |
| -Maintain the unopened kit at 2-8 C. |
| Protocol tips |
-Vigorous washing and complete removal of all liquid by aspiration at the end of each washing step is very important to obtain low background values.
-Dilute the reagents immediately before use. |
| Downstream tips |
-Do not store diluted solutions.
- Maintain the BDNF Standard, at 2-8 C for up to 30 days after reconstitution. |
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