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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours. |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours. |
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Homogenized tissues at 100 mg ml-1 in 1 × phosphate buffered saline+0.5% Triton X-100+protease inhibitor cocktail (Roche, Indianapolis, IN, USA) were centrifuged at 4 °C at 4000 rpm for 5 min. Supernatant was collected and stored at −80 °C. DuoSet ELISA kit determined GDNF concentration (DY212, R&D systems) according to the manufacturer's instructions. Samples were run with biological (n>4 per group) and technical (n=3) replicates. |
|
| Protocol tips |
| Homogenized tissues at 100 mg ml-1 in 1 × phosphate buffered saline+0.5% Triton X-100+protease inhibitor cocktail (Roche, Indianapolis, IN, USA) were centrifuged at 4 °C at 4000 rpm for 5 min. Supernatant was collected and stored at −80 °C. DuoSet ELISA kit determined GDNF concentration (DY212, R&D systems) according to the manufacturer's instructions. Samples were run with biological (n>4 per group) and technical (n=3) replicates. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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