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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
|
| Upstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
| Protocol tips |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Reagents with different lot numbers should not be mixed.
-All reagents need to be
brought to room temperature prior to use. |
-Substrate Solution should remain colourless until added to the plate. Keep Substrate
Solution protected from light .
-Stop Solution should remain colourless until added to the plate. The colour developed in
the wells will turn from blue to yellow immediately after the addition of the Stop Solution.
Wells that are green in colour indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.
|
|
| Upstream tips |
-Reagents with different lot numbers should not be mixed.
-All reagents need to be
brought to room temperature prior to use. |
| Protocol tips |
-Substrate Solution should remain colourless until added to the plate. Keep Substrate
Solution protected from light .
-Stop Solution should remain colourless until added to the plate. The colour developed in
the wells will turn from blue to yellow immediately after the addition of the Stop Solution.
Wells that are green in colour indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.
|
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