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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
|
| Upstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
| Protocol tips |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Do not freeze-thaw the standard and primary antibody more than once. |
-Remove excess wash by gently tapping plate on paper towel or by kimwipe.
-DILUTIONS FOR THE STANDARD CURVE AND
ZERO STANDARD MUST BE MADE AND APPLIED TO THE
PLATE IMMEDIATELY. |
|
| Upstream tips |
| -Do not freeze-thaw the standard and primary antibody more than once. |
| Protocol tips |
-Remove excess wash by gently tapping plate on paper towel or by kimwipe.
-DILUTIONS FOR THE STANDARD CURVE AND
ZERO STANDARD MUST BE MADE AND APPLIED TO THE
PLATE IMMEDIATELY. |
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