ELISA Rat - Transferrin (Tf)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

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Found 3 matching solutions for this experiment

Tf (Rat) ELISA Kit

Abnova

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8°C upon receipt and when it is not in use. -Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air. -All reagents should be allowed to reach room temperature (18-25°C) before use.	-DO NOT DILUTE THE STANDARDS. -Rinse and flick the microtiter wells 5 times with deionized or distilled water. DO NOT USE TAP WATER. -It is recommended that the wells be read within 15 minutes following addition of Stop Solution.

Upstream tips
-Store the unopened kit at 2-8°C upon receipt and when it is not in use. -Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air. -All reagents should be allowed to reach room temperature (18-25°C) before use.
Protocol tips
-DO NOT DILUTE THE STANDARDS. -Rinse and flick the microtiter wells 5 times with deionized or distilled water. DO NOT USE TAP WATER. -It is recommended that the wells be read within 15 minutes following addition of Stop Solution.

Manufacturer protocol Publication protocol 1 Relevant paper

Rat Transferrin ELISA Kit (ab137993)

Abcam

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Rat Transferrin ELISA Kit (Colorimetric)

Novus Biologicals

Upstream tips	Protocol tips	Downstream tips
	-Avoid placing the plate in direct sunlight. -Immediately measure the absorbance of each well using a spectrophotometer/plate reader with the appropriate absorbance setting. -The presence of air bubbles in wells may impact the results and it is advisable to spin the plate in a centrifuge.

Protocol tips
-Avoid placing the plate in direct sunlight. -Immediately measure the absorbance of each well using a spectrophotometer/plate reader with the appropriate absorbance setting. -The presence of air bubbles in wells may impact the results and it is advisable to spin the plate in a centrifuge.

Manufacturer protocol Publication protocol 1 Relevant paper

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