No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 2 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
Heparinized bone marrow (BM) and peripheral blood (PB) cells (106 leukocytes per tube) were incubated with various combinations of mAb (Supplementary Table S3) for 15 minutes. Then, erythrocytes were lysed in FACS-Lysing-Solution (BD Biosciences, San José, CA, USA). Washed cells were acquired on a FACSCalibur (BD Biosciences) and analyzed by FlowJo software (TreeStar, Ashland, OR, USA) |
|
Protocol tips |
Heparinized bone marrow (BM) and peripheral blood (PB) cells (106 leukocytes per tube) were incubated with various combinations of mAb (Supplementary Table S3) for 15 minutes. Then, erythrocytes were lysed in FACS-Lysing-Solution (BD Biosciences, San José, CA, USA). Washed cells were acquired on a FACSCalibur (BD Biosciences) and analyzed by FlowJo software (TreeStar, Ashland, OR, USA) |
Upstream tips |
Protocol tips |
Downstream tips |
|
Mononuclear cells were analyzed via flow cytometry (CyFlow Space, Partec) immediately after their isolation from blood. |
. At least 1 × 106 cells were stained for each antibody, with at least 5 × 105 events captured and analyzed. Unstained and single stain controls were used for compensation and gating. Final gates were based on FSC/SSC and two parameter plots. |
Protocol tips |
Mononuclear cells were analyzed via flow cytometry (CyFlow Space, Partec) immediately after their isolation from blood. |
Downstream tips |
. At least 1 × 106 cells were stained for each antibody, with at least 5 × 105 events captured and analyzed. Unstained and single stain controls were used for compensation and gating. Final gates were based on FSC/SSC and two parameter plots. |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!