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Found 2 matching solutions for this experiment
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neurospheres were dissociated into a single cell suspension using Accutase (Innovative Cell Technologies) and prepared for fluorescence activated cell sorting (FACS) analysis |
Cells were analyzed using an LSR II Flow Cytometer or FACSCalibur (Becton Dickinson Biosciences) by the UAB Flow Cytometry Core Facility, and the results were expressed as a percentage of gated cells based on antibody binding using FlowJo version 10.0.6 software (Tree Star). |
Protocol tips |
neurospheres were dissociated into a single cell suspension using Accutase (Innovative Cell Technologies) and prepared for fluorescence activated cell sorting (FACS) analysis |
Downstream tips |
Cells were analyzed using an LSR II Flow Cytometer or FACSCalibur (Becton Dickinson Biosciences) by the UAB Flow Cytometry Core Facility, and the results were expressed as a percentage of gated cells based on antibody binding using FlowJo version 10.0.6 software (Tree Star). |
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1:100 for anti–nectin-1 antibody (Zymed) |
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1:100 for anti–nectin-1 antibody (Zymed) |
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