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Found 2 matching solutions for this experiment
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For flow cytometry analysis, cultured cells were trypsinized and resuspended in in PBS with 2% FBS and 5 mM EDTA, and stained with primary antibody for 30 min at 4 °C. |
Stained cells were analyzed by FACSCantoTM II (BD Biosciences). |
Protocol tips |
For flow cytometry analysis, cultured cells were trypsinized and resuspended in in PBS with 2% FBS and 5 mM EDTA, and stained with primary antibody for 30 min at 4 °C. |
Downstream tips |
Stained cells were analyzed by FACSCantoTM II (BD Biosciences). |
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Cytometry and sorting was performed using a BD FACSAria (Becton Dickinson). |
Downstream tips |
Cytometry and sorting was performed using a BD FACSAria (Becton Dickinson). |
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