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Found 3 matching solutions for this experiment
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neurospheres were dissociated into a single cell suspension using Accutase (Innovative Cell Technologies) and prepared for fluorescence activated cell sorting (FACS) analysis as we have previously described. |
Cells were analyzed using an LSR II Flow Cytometer or FACSCalibur (Becton Dickinson Biosciences) by the UAB Flow Cytometry Core Facility, and the results were expressed as a percentage of gated cells based on antibody binding using FlowJo version 10.0.6 software (Tree Star). |
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neurospheres were dissociated into a single cell suspension using Accutase (Innovative Cell Technologies) and prepared for fluorescence activated cell sorting (FACS) analysis as we have previously described. |
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Cells were analyzed using an LSR II Flow Cytometer or FACSCalibur (Becton Dickinson Biosciences) by the UAB Flow Cytometry Core Facility, and the results were expressed as a percentage of gated cells based on antibody binding using FlowJo version 10.0.6 software (Tree Star). |
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The cultured glioma cells were digested by trypsin or accutase and resuspended with PBS. The fresh glioma specimens were transferred to laboratory on ice in half hour after surgery, then washed and enzymatically dissociated into single cells and resuspended in PBS. |
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The FACS analysis and cell sorting were performed on BD FACS Aria II cytometer (USA) or Beckman moflo XDP (USA). |
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The cultured glioma cells were digested by trypsin or accutase and resuspended with PBS. The fresh glioma specimens were transferred to laboratory on ice in half hour after surgery, then washed and enzymatically dissociated into single cells and resuspended in PBS. |
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The FACS analysis and cell sorting were performed on BD FACS Aria II cytometer (USA) or Beckman moflo XDP (USA). |
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>20 × 105 disaggregated sphere cells were stained with a stem cell‐specific antibody panel and analysed on a BDTM LSR II flow cytometer. To a volume of 100 μL cell suspension, 5 μL antibody mix was added, mixed and incubated for 20 minutes at 4°C in the dark and washed twice with 250 μL DPBS or FACS buffer. |
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>20 × 105 disaggregated sphere cells were stained with a stem cell‐specific antibody panel and analysed on a BDTM LSR II flow cytometer. To a volume of 100 μL cell suspension, 5 μL antibody mix was added, mixed and incubated for 20 minutes at 4°C in the dark and washed twice with 250 μL DPBS or FACS buffer. |
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