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Found 3 matching solutions for this experiment
Upstream tips |
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A total of 0.1 ml of peripheral blood was incubated with 10 μl of antibody for 15 min at room temperature, then erythrocytes were lysed and leucocytes post-fixed. Afterwards, the flow cytometry analysis was performed. |
Flow cytometric analysis was performed using a Navios Flow Cytometer and the Kaluza analysis software (Beckman Coulter, Milan, Italy), evaluating a total of 5 × 106 cells and detecting more than 30 events in the smallest subset investigated, according to consensus guidelines on the minimal residual disease |
Protocol tips |
A total of 0.1 ml of peripheral blood was incubated with 10 μl of antibody for 15 min at room temperature, then erythrocytes were lysed and leucocytes post-fixed. Afterwards, the flow cytometry analysis was performed. |
Downstream tips |
Flow cytometric analysis was performed using a Navios Flow Cytometer and the Kaluza analysis software (Beckman Coulter, Milan, Italy), evaluating a total of 5 × 106 cells and detecting more than 30 events in the smallest subset investigated, according to consensus guidelines on the minimal residual disease |
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All samples were analyzed using FlowJo Version 10 software (Ashland, OR). Forward scatter-area (FSC-A) and side scatter-area (SSC-A) gates were initially set based on size and density using control sample tissues. |
Downstream tips |
All samples were analyzed using FlowJo Version 10 software (Ashland, OR). Forward scatter-area (FSC-A) and side scatter-area (SSC-A) gates were initially set based on size and density using control sample tissues. |
Upstream tips |
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Downstream tips |
Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages |
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Upstream tips |
Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages |
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