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Found 3 matching solutions for this experiment
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The cells were concentrated by centrifugation for 240 seconds at 1200 g and after discarding the supernatant, 100 microliters (μL) of the cell suspension were stained using 5 μL of CD326 (clone Ber‐Ep4, DAKO, Denmark) conjugated with fluorescein isothiocyanate (FITC) |
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The cells were concentrated by centrifugation for 240 seconds at 1200 g and after discarding the supernatant, 100 microliters (μL) of the cell suspension were stained using 5 μL of CD326 (clone Ber‐Ep4, DAKO, Denmark) conjugated with fluorescein isothiocyanate (FITC) |
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Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C. |
Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences). |
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Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C. |
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Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences). |
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All stained cell suspensions were detected or sorted using an AccuriC6 cytometer (BD Biosciences) and analyzed by FlowJo 7.6.1. |
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All stained cell suspensions were detected or sorted using an AccuriC6 cytometer (BD Biosciences) and analyzed by FlowJo 7.6.1. |
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