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Found 3 matching solutions for this experiment
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>20 × 105 disaggregated sphere cells were stained with a stem cell‐specific antibody panel and analysed on a BDTM LSR II flow cytometer. To a volume of 100 μL cell suspension, 5 μL antibody mix was added, mixed and incubated for 20 minutes at 4°C in the dark and washed twice with 250 μL DPBS or FACS buffer. |
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>20 × 105 disaggregated sphere cells were stained with a stem cell‐specific antibody panel and analysed on a BDTM LSR II flow cytometer. To a volume of 100 μL cell suspension, 5 μL antibody mix was added, mixed and incubated for 20 minutes at 4°C in the dark and washed twice with 250 μL DPBS or FACS buffer. |
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Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C. |
Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences). |
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Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C. |
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Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences). |
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Fluorescence-activated cell sorting BD, FACSAria II SORP, and FACSDiva 8.0.1 software were used. |
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Fluorescence-activated cell sorting BD, FACSAria II SORP, and FACSDiva 8.0.1 software were used. |
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