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3 years ago
3 years ago by M. Daecher
Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
Found 2 matching solutions for this experiment
|Remove the supernatant and resuspend the pellet of one tube in 0.3 ml of 0.85% NaCl solution and another tube in 1 ml of 0.85% NaCl.
Add 0.7 ml isopropyl alcohol into the tube with 0.3 ml of 0.85% NaCl and mix well
Incubate both samples at room temperature for 1 hour, mixing every 15 minutes.
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