Found 1 discussion for this experiment
3 years ago
3 years ago by M. Daecher
Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
Found 1 matching solution for this experiment
|Remove the supernatant and resuspend the pellet of one tube in 0.3 mL of 0.85% NaCl solution and another tube in 1 mL of 0.85% NaCl.
Add 0.7 mL isopropyl alcohol into the tube with 0.3 mL of 0.85% NaCl and mix well (final concentration of isopropyl alcohol: 70%) for preparing dead bacteria.
Incubate both samples at room temperature for 1 hour, mixing every 15 minutes
Fill out your contact details and receive price quotes in your InboxOutsource experiment