Live / Dead assay mammalian cells - INS-1 832/12

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Protocol tips
Add 16 uL of CellTiter-Blue dye to wells containing 80 μL of cell culture media and incubate cells in a cell culture incubator at 37°C and 5% CO2 for 1 hour
Protocol tips
Add 10 μl of the CCK-8 solution to each well of the plate and incubate the plate for 1hour in the incubator
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