Live / Dead assay mammalian cells - K562

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Upstream tips
Incubate cells in staining solution overnight under normal
culture conditions.
Protocol tips
Effector cells were added at effector-to-target (E:T) ratios of 1.25:1, 2.5:1, 5:1, 10:1, 20:1, 40:1, and 80:1.
Protocol tips
Add 1 × RealTime-Glo MT Cell Viability Assay reagent to the culture
Downstream tips
Luminescence was read on a Tecan Infinite M200 plate reader at 3, 6, 12, 22, 31, and 47 h.
Protocol tips
Mix 2μM calcein AM and and 4 μM propidium iodide and incubate the mixture for 15 minutes at 37C
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