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Found 3 matching solutions for this experiment
|Add the MTT Reagent to each well at a 1:10 ratio. For example, add 10 µL/well for a 96-well
plate or 25 µL/well for a 24-well plate.
Incubate the wells 2-4 hours or overnight at 37°C. Monitor the cells occasionally with an
inverted microscope for the presence of a purple precipitate
|Add Calcein AM (0.5 μM) and ethidium homodimer-1 (1 μM)
Incubate the cells for 30–45 minutes at room temperature
|Add 100 µl of assay solution to cells and incubate the mixture at 37 ºC for 15 min.
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