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|Add 4 μM of calcein acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide.
Incubate cells for 30-40 min at room temperature in the dark.
|It is recommended to analyze the cells as quickly after
staining as possible. The staining solution should not be exchanged for another buffer before data acquisition. Stained cells are stable for at least one hour at room
temperature after staining. Live and Dead dye staining is lost if cells are fixed.