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Found 3 matching solutions for this experiment
|Don’t use Tris buffer as a diluent and be sure that the PBS does not contain any other protein
like BSA or FBS
|Dilute Zombie UV™ dye at 1:100-1000 in PBS.
Incubate the cells at room temperature, in the dark, for 15-30 minutes
|Add 1 μL of the reconstituted fluorescent reactive dye.
Incubate at room temperature or on ice for 30 minutes, protected from light.
|Detected at ~450 nm recommended filters (450/50 nm or similar)
|Add reconstituted fluorescent reactive dye and incubate at room temperature or on ice for 30 minutes
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