Live / Dead assay mammalian cells - mouse, T-cell

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 1 matching solution for this experiment

LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Add 1 μL of the reconstituted fluorescent reactive dye. Incubate at room temperature or on ice for 30 minutes, protected from light.	Detect at ~525 nm and use filters 525/50 nm or similar.

Protocol tips
Add 1 μL of the reconstituted fluorescent reactive dye. Incubate at room temperature or on ice for 30 minutes, protected from light.
Downstream tips
Detect at ~525 nm and use filters 525/50 nm or similar.

Manufacturer protocol Publication protocol 1 Relevant paper

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