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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Prepare a staining solution of 2 µM Calcein-AM/4 µM EthD-III by adding 5 µl
of 4 mM Calcein-AM and 20 µl of 2 mM EthD-III to 10 ml of PBS or other serum-free buffer or medium. |
Incubate the cells for 15 minutes at room temperature |
|
| Upstream tips |
Prepare a staining solution of 2 µM Calcein-AM/4 µM EthD-III by adding 5 µl
of 4 mM Calcein-AM and 20 µl of 2 mM EthD-III to 10 ml of PBS or other serum-free buffer or medium. |
| Protocol tips |
| Incubate the cells for 15 minutes at room temperature |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Collect cells by centrifugation and resuspend to 0.5 ml Staining Solution.
Incubate for 15 min at 37°C |
|
| Protocol tips |
Collect cells by centrifugation and resuspend to 0.5 ml Staining Solution.
Incubate for 15 min at 37°C |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Add 5 μL of 4 mM calcein-AM solution.
Incubate the cells for 45 minutes at room temperature. |
|
| Protocol tips |
Add 5 μL of 4 mM calcein-AM solution.
Incubate the cells for 45 minutes at room temperature. |
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