Live / Dead assay mammalian cells - rat testicular tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 1 matching solution for this experiment

LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Add the LIVE/DEAD Near‐IR stain reconstituted in DMSO to the cells at a final concentration of 1 : 10 000 Incubate for 30 min on ice and pellet by centrifugation (400 g, 10 min at +4 °C) and resuspend in 1 mL of PBS.	Detect at ~780 nm.

Protocol tips
Add the LIVE/DEAD Near‐IR stain reconstituted in DMSO to the cells at a final concentration of 1 : 10 000 Incubate for 30 min on ice and pellet by centrifugation (400 g, 10 min at +4 °C) and resuspend in 1 mL of PBS.
Downstream tips
Detect at ~780 nm.

Manufacturer protocol Publication protocol 1 Relevant paper

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