Live / Dead assay mammalian cells - RAW 264.7

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Protocol tips
Add the 2X MultiTox-Fluor Multiplex Cytotoxicity Assay Reagent in an
equal volume to all wells.

Mix briefly on an orbital shaker,then incubate for at least 30 minutes at 37°C.

Do not incubate longer than 3 hours
Protocol tips
Incubate the cells in an incubator (37°C, 5% CO2, 90% humidity)
LIVE/DEAD™ Cell Imaging Kit

Thermo Fisher Scientific

Protocol tips
Incubate for 15 minutes at
20–25°C
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