Live / Dead assay mammalian cells - THP-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Protocol tips
Add an equal volume of 2X RealTime-Glo™ reagent to the cells.

Incubate the cells for 10–60 minutes in the cell culture incubator
Protocol tips
Add 1mL of calcein.

Incubate the cells for 15–20 minutes at room temperature,
protected from light
Protocol tips
Incubate for 15 min at 37 ° C
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