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Found 2 matching solutions for this experiment
| Add 1 µL of Component A (FUN® 1 cell stain) and 5 µL of Component B (Calcofluor® White M2R) to 1 mL of yeast suspension (10 µM and 25 µM final concentrations of each of the dyes, respectively).
Incubate at 30°C in the dark for 30 minutes
|Add 1 µL of Component SYTO and 1 µL of Component propidium iodide.
After stain is added, each tube should be vortexed gently.
Incubate all samples at room temperature or 37ºC protected from light for 15–30 minutes
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