Live / Dead assay yeast - Urediniospore

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

LIVE/DEAD™ Yeast Viability Kit

Thermo Fisher Scientific

Protocol tips
Add 1 µL of Component A (FUN® 1 cell stain) and 5 µL of Component B (Calcofluor® White M2R) to 1 mL of yeast suspension (10 µM and 25 µM final concentrations of each of the dyes, respectively).

Incubate at 30°C in the dark for 30 minutes
Protocol tips
Add 1 µL of Component SYTO and 1 µL of Component propidium iodide.

After stain is added, each tube should be vortexed gently.

Incubate all samples at room temperature or 37ºC protected from light for 15–30 minutes
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms