Mammalian cell culture media A253

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Protocol tips
The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.
Protocol tips
supplemented with 10% fetal bovine serum (Welgene) and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA) at 37°C in a humidified 5% CO2 incubator.
Gibco™ RPMI 1640 Medium

Fisher Scientific

Protocol tips
supplemented with 10% fetal bovine serum (GIBCO-BRL, Life Technologies).
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