Mammalian cell culture media BEnEpC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 2 matching solutions for this experiment

RPMI-1640 Medium

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	culture media containing 10% FBS, penicillin (50 IU/ml; Sigma), streptomycin (50 μg/ml; Sigma) and amphotericin B (2.5 μg/ml; Sigma) in RPMI 1640 (Sigma)

Protocol tips
culture media containing 10% FBS, penicillin (50 IU/ml; Sigma), streptomycin (50 μg/ml; Sigma) and amphotericin B (2.5 μg/ml; Sigma) in RPMI 1640 (Sigma)

Manufacturer protocol Publication protocol 1 Relevant paper

Gibco DMEM/F-12, HEPES

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	B-27 Supplement (Gibco, 17504-044, 50×), N-2 Supplement (Gibco, 17502-048, 100×) and Glutamax (Gibco, 35050-061, 100×), with mixed feeders

Protocol tips
B-27 Supplement (Gibco, 17504-044, 50×), N-2 Supplement (Gibco, 17502-048, 100×) and Glutamax (Gibco, 35050-061, 100×), with mixed feeders

Manufacturer protocol Publication protocol 1 Relevant paper

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