Mammalian cell culture media HC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

BIO-MPM-1 SFM

Biological Industries

Upstream tips	Protocol tips	Downstream tips
	Monolayer cultures were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 50 U/ml penicillin, and 50 μg/ml streptomycin (Biologic Industries, Beit-Haemek Kibutz, Israel). The cells were treated with the proinflammatory cytokines IL1β (5 ng/ml) and TNF-α (50 ng/ml; both from Peprotec, Rehovot, Israel) in defined serum-free medium (BIO-MPM 1; Biologic Industries, Beit-Haemek Kibutz, Israel).

Protocol tips
Monolayer cultures were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 50 U/ml penicillin, and 50 μg/ml streptomycin (Biologic Industries, Beit-Haemek Kibutz, Israel). The cells were treated with the proinflammatory cytokines IL1β (5 ng/ml) and TNF-α (50 ng/ml; both from Peprotec, Rehovot, Israel) in defined serum-free medium (BIO-MPM 1; Biologic Industries, Beit-Haemek Kibutz, Israel).

Protocol tips

Monolayer cultures were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 50 U/ml penicillin, and 50 μg/ml streptomycin (Biologic Industries, Beit-Haemek Kibutz, Israel). The cells were treated with the proinflammatory cytokines IL1β (5 ng/ml) and TNF-α (50 ng/ml; both from Peprotec, Rehovot, Israel) in defined serum-free medium (BIO-MPM 1; Biologic Industries, Beit-Haemek Kibutz, Israel).

Manufacturer protocol Publication protocol 1 Relevant paper

Advanced DMEM

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Advanced DMEM supplemented with 100 IU/ml Penicillin, 1 mg/ml Streptomycin, 2mM L-glutamine and 5 wt.% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA)

Protocol tips
Advanced DMEM supplemented with 100 IU/ml Penicillin, 1 mg/ml Streptomycin, 2mM L-glutamine and 5 wt.% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA)

Manufacturer protocol Publication protocol 1 Relevant paper

Growth Medium SupplementMix

PromoCell

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum and 1% penicillin(HyClone; Thermo Fisher Scientific, Waltham, MA, USA).

Protocol tips
supplemented with 10% fetal bovine serum and 1% penicillin(HyClone; Thermo Fisher Scientific, Waltham, MA, USA).

Manufacturer protocol Publication protocol 1 Relevant paper

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