Mammalian cell culture media HCAEC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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EBMTM-2 Endothelial Cell Growth Basal Medium-2

Lonza

Upstream tips	Protocol tips	Downstream tips
	Human coronary artery endothelial cells (HCAECs), endothelial basal medium-2 (EBM-2), EGM-2-MV SingleQuots, fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were purchased from Lonza (Viviere, Belgium). HCAECs were maintained in EBM-2 supplemented with EGM-2-MV SingleQuots (containing vascular endothelial growth factor, basic fibroblast growth factor, insulin-like growth factor-I, epidermal growth factor, ascorbic acid, and gentamicin), and 10% FBS. All cells were cultured at 37°C in an incubator with a humidified atmosphere and 5% CO.

Protocol tips

Human coronary artery endothelial cells (HCAECs), endothelial basal medium-2 (EBM-2), EGM-2-MV SingleQuots, fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were purchased from Lonza (Viviere, Belgium). HCAECs were maintained in EBM-2 supplemented with EGM-2-MV SingleQuots (containing vascular endothelial growth factor, basic fibroblast growth factor, insulin-like growth factor-I, epidermal growth factor, ascorbic acid, and gentamicin), and 10% FBS. All cells were cultured at 37°C in an incubator with a humidified atmosphere and 5% CO.

Manufacturer protocol Publication protocol 1 Relevant paper

Vascular Cell Basal Medium

LGC Standards

Upstream tips	Protocol tips	Downstream tips
	Vascular cell basal media (#PCS-100-030; ATCC) supplemented with 0.2% bovinebrain extract, 5 ngmL1 human epidermal growth factor,10 mM L-glutamine, 0.75 unitsmL1 heparin sulfate,1 lgmL1 hydrocortisone, 50 lgmL1 ascorbic acid, 2%fetal bovine serum and pen/strep.

Protocol tips
Vascular cell basal media (#PCS-100-030; ATCC) supplemented with 0.2% bovinebrain extract, 5 ngmL1 human epidermal growth factor,10 mM L-glutamine, 0.75 unitsmL1 heparin sulfate,1 lgmL1 hydrocortisone, 50 lgmL1 ascorbic acid, 2%fetal bovine serum and pen/strep.

Manufacturer protocol Publication protocol 1 Relevant paper

Human MesoEndo Cell Growth Medium

Cell Applications Inc

Upstream tips	Protocol tips	Downstream tips
	Primary human coronary artery endothelial cells (HCAEC; purchased from Cell Applications, San Diego, CA, USA) were cultured (37°C, 5% CO2) in MesoEndo medium (Cell Applications).

Protocol tips
Primary human coronary artery endothelial cells (HCAEC; purchased from Cell Applications, San Diego, CA, USA) were cultured (37°C, 5% CO2) in MesoEndo medium (Cell Applications).

Manufacturer protocol Publication protocol 1 Relevant paper

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